ABSTRACT
Objective: this study was performed to analyze the morphological changes in primary oocyte and zona pellucida of primary follicle in prepubertal albino rats of three weeks ago after exposure to gamma radiation
Material and Methods: 72 female albino rats of age of three weeks were used. Out of these 24, received 4.5 Gray, another 24 received 8.3 Gray of Gamma radiation, and the rest of them received sham radiation. Ovaries of rats were dissected after one day, 7 days and 14 days after irradiation. Five random histological sections of each ovary were examined
Results: intense necrosis was evident in oocyte and granulosa cells of primary follicles after one day- post radiation. The zona pellucid a remained in contact with the oocyte and granulosa cells in initial phase of atresia. With progressing atresia, zona pellucida lost its contact with the oocytes and granulosa cells. Disintegration of zona pellucida started after development of apoptotic bodies in primary follicles. Disintegrated zona pellucida disappeared rapidly in experimental groups, particularly in high dose group. The basement membrane was irregular and disrupted in atretic follicles
Conclusion: therefore, we can conclude that gamma radiation induces rapid necrosis and cyst formation in primary oocyte. Disintegration of zona pellucida with progression of atresia in ovarian follicle was evident. Gamma radiation also produces irregular, disrupted and thinner basement membrane
ABSTRACT
Twelve adult and 48 old albino rats were arranged from Veterinary Reseach Institute Herike Road, Lahore to study the efficacy of steroid, androgen and combined therapy of both drugs [30 days] for reversion of senile prostate hypertrophy to its normal structure and function. Macro and Microscopically, it was observed that testosterone alone or in combination with methylprednisolone showed capability for reduction/reversion of senile enlargement in prostate gland to its normal architecture and secretion